VereVet™

VereVet™ is a nucleic acid-based, Lab-On-Chip (LOC) device which combines multiplex PCR and microarray hybridization to detect, differentiate and identify multiple Avian Pathogens in one test.

Avian diseases are rapidly becoming an international health and industry concern due to its ease of spread and inherent potential to cross infect humans.

Firstly, avian diseases can be transmitted from one flock to the other easily, causing the poultry industry to suffer losses in the form of reduced meat and egg production from sick or dead birds.

Secondly, other than disease transmission within and between flocks, avian diseases have also been known to cross the species barrier to infect humans and have thus caused four pandemics in the last century1. The extent of impact can be seen clearly in cases of avian influenza outbreaks, where certain strains have been found to be very contagious and lethal within the species, and have been known to transfer its high infection rate and lethal attributes when the same strains infect humans, stressing the need for the health authorities to implement quarantine measures.

These problems associated with avian diseases clearly indicate the necessity for a tool that enables early detection, containment and treatment of diseases in a flock through accurate and prompt identification, hence protecting livestock and humans.

To meet this need, Veredus has developed VereVet™, a multiplexed assay that utilizes the VerePLEX™ Lab-on-Chip platform to detect, differentiate and identify 9 major avian pathogens and subtypes.

For Research Use Only. Not for use in diagnostic procedures.

1Institute for Animal Health, http://www.pirbright.ac.uk/ecosoc/docs/DTZ%20Avian%20Research%20Impact.pdf

Detects Multiple Avian Pathogens

Multiple probes (with duplicates) for:

  • Avian Influenza (type probes for Flu A, H5, H7, H9, N1)
  • Newcastle Disease Virus (NDV) matrix gene
  • Salmonella pullorum
  • Salmonella enteritidis
  • Campylobacter spp.

Specificity: 93.3 – 100%

Limit of Detection: 100 copies of DNA/RNA

Sample Types: Tracheal swabs, cloacal swabs, fecal swabs and fecal samples

 

Speed

  • Fast turnaround time––sample to answer in less than 3 hours to provide multiple results versus 2 or more days for culture-based testing
  • Rapid detection of avian pathogens enables prompt actions by authorities to contain, treat and control disease outbreaks

Comprehensive

  • Tests for selected generic and specific avian pathogens in a single assay

Easy to use

  • The simple workflow allows for minimally trained or non-scientific personnel to run tests

Updates Available

Probes can be updated quickly to include new mutations of evolving strains and ensure wider coverage of detection

  • Multiplex amplification reactions
  • Multiple probes per target ensures reliable detection of subtypes in every test
  • Small sample volume requirement
  • Fast and programmable temperature ramp rate
  • Scalable for high throughput
  • PCR yield is comparable to standard thermal cyclers
  • 40% faster than conventional thermal cyclers
  • Functional validation of PCR is provided by an internal positive control
  • Functional validation of hybridization for each assay is provided by an internal positive hybridization control
  • Proprietary microfluidic interface: contact surfaces are biocompatible and do not inhibit the PCR reaction
  • Short time required for fluidic operations

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